Composite
Aad9-T

Part:BBa_K143065:Design

Designed by: Chris Hirst and Imperial iGEM 08   Group: iGEM08_Imperial_College   (2008-10-09)

Spectinomycin Resistance Protein (Aad9) - Terminator



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488

Design Notes

Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source

Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry